FIGURE

Fig. 2

ID

ZDB-FIG-230405-9

Publication

Tan et al., 2022 - Pax2a, Sp5a and Sp5l act downstream of Fgf and Wnt to coordinate sensory-neural patterning in the inner ear

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Fig. 2

Effects of altered Wnt and Fgf signaling on sensory-neural patterning in the otic vesicle. (A) Dorsolateral views (anterior to the left) showing expression of otic markers of the medial wall, sensory epithelia and neurogenic domains (indicated on the left) at 24 hpf following treatments to alter Wnt and Fgf signaling at 12 hpf. Oval marquees encircle the otic vesicle. Mean anteroposterior length of the otic vesicle (±SD, n ≥ 8) is indicated in images of pax2a expression (Aa-e). Scale bar (Aa), 50 μm. (B) Dorsolateral views of anti-Isl1/2 stained SAG neurons at 30 hpf (a–d) and brn3c:Gfp + hair cells at 36 hpf (e–h) under the indicated conditions. (C, D) Quantification of the effects of signal modulation under conditions indicated by the key at the top. Mean values with errors (standard deviations) and sample sizes are indicated for each group. Asterisks indicate significant differences relative to controls. (C) Mean number of anti-Isl1/2 stained SAG neurons at 30 hpf. (D) Mean number of brn3c:gfp + hair cells in the anterior and posterior sensory epithelia at 30 and 36 hpf.

Expression Data

Genes:	atoh1a GFP gsc neurog1 pax2a sp5a sp5l
Antibody:	Ab2-isl
Fish:	s356tTg/s356tTg AB w32Tg/w32Tg x17Tg/x17Tg s356tTg/s356tTg; w32Tg/w32Tg s356tTg/s356tTg; x17Tg/x17Tg
Conditions:	chemical treatment by environment: 6-bromoindirubin-3'-oxime chemical treatment by environment: IWR-1-endo chemical treatment by environment: SU5402
Anatomical Terms:	otic vesicle otic vesicle anterior region otic vesicle hair cell otic vesicle medial region otic vesicle neuron
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s356tTg/s356tTg AB w32Tg/w32Tg x17Tg/x17Tg s356tTg/s356tTg; w32Tg/w32Tg
Conditions:	chemical treatment by environment: 6-bromoindirubin-3'-oxime chemical treatment by environment: IWR-1-endo chemical treatment by environment: SU5402
Observed In:	otic vesicle otic vesicle anterior region otic vesicle hair cell otic vesicle medial region otic vesicle neuron
Stage Range:	Prim-5 to Prim-25

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 492, Tan, A.L., Mohanty, S., Guo, J., Lekven, A.C., Riley, B.B., Pax2a, Sp5a and Sp5l act downstream of Fgf and Wnt to coordinate sensory-neural patterning in the inner ear, 139-153, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.