FIGURE

Figure 3

ID

ZDB-FIG-230321-9

Publication

Berger et al., 2023 - The Role of TRiC-enhanced Actin Folding in Leber Congenital Amaurosis

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Figure 3

F-actin is significantly reduced in the retina of cct5^{𝑡𝑓121𝑏} mutants. (A) At three dpf, F-actin was labelled with phalloidin (red) on cross sections counterstained with DAPI (blue). Whereas the inner and outer plexiform layers of the retina (IPL and OPL, respectively) were prominently stained by phalloidin within siblings, the signal from cct5^{𝑡𝑓121𝑏} homozygotes appeared weaker. Phalloidin also marked the optic nerve (ON). (B) Quantification of the brightness of the phalloidin signal within the IPL revealed that cct5^{𝑡𝑓121𝑏} homozygotes showed a significant reduction in signal intensity to 54 ± 4% in relation to their siblings, which were rescaled to 100 ± 3%. Data are mean ± SEM; ***P < 0.001 by Student’s t-test; n = 10.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cct5^{tf212b/tf212b} (TU)
Observed In:	retinal inner plexiform layer filamentous actin retinal outer plexiform layer filamentous actin
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Ophthalmic Vis. Res.