Fig. 3.

A two-phase acquisition protocol to combine acquisition of quality control images with high-speed time-lapse imaging. (A) Image data were acquired at multiple positions in a sample, thus obtaining multiple viewpoints containing several objects of interest (nuclei, indicated as circles). (B) For each position, a sequence of two acquisition phases is carried out. In phase A, for each z position, a low-quality image, two high-quality reference images, and two low-quality test images are recorded. Low-quality images are recorded at a shortened exposure time (t_exp), high-quality images at a reference exposure time resulting in images of the desired quality (t_ref). Acquisition phase A obtains the images required for n2v model training as well as the assessment of effective image resolution and reconstruction errors. In phase B, only single low-quality images are recorded with the shortened exposure time (t_exp), resulting in an increased rate of acquisition compared to acquisition with full exposure time (t_ref). Acquisition phase B obtains only low-quality images, which are reconstructed after the experiment is completed.