FIGURE

Figure 4

ID

ZDB-FIG-210915-91

Publication

Schellens et al., 2021 - Zebrafish as a Model to Evaluate a CRISPR/Cas9-Based Exon Excision Approach as a Future Treatment Option for EYS-Associated Retinitis Pigmentosa

Other Figures

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Figure 4

Transcript analysis of eys^Δexon40-44 mRNA. (A) RT-PCR analysis revealed the absence of eys exons 40-44 in eys^Δexon40-44 larvae (5 days post fertilization (dpf)), in the absence of any clear alternatively spliced eys transcripts. (B) Sanger sequencing of the eys^Δexon40-44 amplicon confirmed the absence of exons 40-44 from the transcript. (C) Long-range PCR analysis did not reveal any additional and unwanted amplicons derived from CRISPR/Cas9-induced alternative splicing of eys transcripts. PCR (-): negative PCR control.

Expression Data

Gene:	eys
Fish:	TL eys^rmc9/rmc9 (TL)
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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