IMAGE

Figure 4

ID

ZDB-IMAGE-210915-91

Genes

Source

Figures for Schellens et al., 2021

Image

Figure Caption

Figure 4

Transcript analysis of eys^Δexon40-44 mRNA. (A) RT-PCR analysis revealed the absence of eys exons 40-44 in eys^Δexon40-44 larvae (5 days post fertilization (dpf)), in the absence of any clear alternatively spliced eys transcripts. (B) Sanger sequencing of the eys^Δexon40-44 amplicon confirmed the absence of exons 40-44 from the transcript. (C) Long-range PCR analysis did not reveal any additional and unwanted amplicons derived from CRISPR/Cas9-induced alternative splicing of eys transcripts. PCR (-): negative PCR control.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.