Fig 2

A. Maternal igf2bp3^Δ7 mutants show severe defects by blastula and gastrula stages. B. At the 64-cell stage, most maternal igf2bp3^Δ7 mutants are similar to wild type embryos (left), but a proportion of embryos (right; <15%) show rounded cells (red arrowheads). C-D. The majority of igf2bp3 mutants do not survive beyond 1 dpf. E. Animal-vegetal markers (gdf3, dazl and wnt8a) show altered distribution in igf2bp3^Δ7 embryos by whole mount in situ hybridization (WISH). F. qRT-PCRs show that expression levels of gdf3, dazl and wnt8a transcripts is not significantly different from wild type control embryos at the 1-cell stage. G-I. qRT-PCR with maternal igf2bp3^Δ7 embryos show that expression of igf2bp3 transcripts is significantly reduced at all stages examined (1 cell, 1K, and 50% epiboly), whereas expression of the yolk syncytial layer (YSL) transcript mxtx2 is initially unchanged (G), but shows variable increase at the 1K stage (F), with substantially higher expression at 50% epiboly compared to controls (I), and the microtubule and spectrin-associated camsap3 transcript is largely unchanged at all stages examined. p ***<0.01 or ****<0.001 from 3 biological replicates. Scale bar in A, 200 μm.