FIGURE

Fig 3

ID

ZDB-FIG-210801-42

Publication

Vong et al., 2021 - The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish

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Fig 3

MZ<italic toggle='yes'>igf2bp3</italic><sup><italic toggle='yes'>la659Tg</italic></sup> mutant embryos show premature translation of a Nodal reporter.

A. Schematic of the sqt-gfp fusion reporter construct showing the Sqt pro-domain, the enterokinase cleavage site, 3xFLAG epitope tag, eGFP and mature Nodal peptide sequences. Expression of a Sqt-GFP reporter expression (green) in MZigf2bp3^la659Tg embryos compared to wild type or control heterozygous embryos. Representative examples from three independent experiments are shown, imaged at the 1K, high or sphere stage, respectively. Scale bar, 50 μm. B. Bar charts represent mean GFP intensity in the blastoderm of imaged embryos. GFP signals were normalized to co-injected rhodamine dextran control. Number of embryos analysed: 1K (n = 9 WT and 10 MZigf2bp3^la659Tg), high (n = 10 Control and 11 MZigf2bp3^la659Tg), sphere (n = 9 WT and 9 MZigf2bp3^la659Tg). Asterisks indicate level of significance from two-tailed t tests (p *<0.05, **<0.01, ***<0.001).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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