FIGURE

Fig. 5

ID
ZDB-FIG-210520-5
Publication
Bertho et al., 2021 - Zebrafish dazl regulates cystogenesis and germline stem cell specification during the primordial germ cell to germline stem cell transition
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Fig. 5

Single confocal plane of representative dazlΔ7/+ or dazlΔ7/Δ7 day 8 gonads and dazlae57/+, dazlΔ7/+ or dazlΔ7/ae57 at 10 days labeled with Vasa (green), LaminB1 (magenta; nuclear envelope,) and DAPI (gray; DNA). (A-A″) Vasa+ GC of dazlΔ7/+ (n=2) at day 8, with a folded raisin-like nuclear envelope. (B-B″) Vasa+ GC of dazlΔ7/Δ7 (n=3) at day 8, with an unfolded smooth nuclear envelope of Vasa+ GC of dazlae57/+ (C-C″, n=2) at 10 days. (D-D″) The change in nuclear appearance corresponds to amplification during cyst formation and is intact in dazlae57/Δ7. (E-E″) After the transition at 10 days, the nuclei of Vasa+ GC of dazlΔ7/+ remain smooth (n=2). The postmitotic GC nuclei also have a smooth nuclear membrane and a large nucleolus. (F-F″) Vasa+ GC of dazlae57/Δ7 (n=5) at 10 days. Mutant GCs are individuals with a folded nuclear membrane rather than a smooth morphology at 10 days. Yellow dotted line delineates the gonad. Blue arrows indicate each note for the corresponding panel.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Days 7-13

Phenotype Detail
Acknowledgments
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