FIGURE

Figure 6.

ID

ZDB-FIG-210512-33

Publication

Zhang et al., 2021 - Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency

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Figure 6.

The instability of OXPHOS complexes. (A) The steady-state levels of five OXPHOS complexes by Blue-Native gel electrophoresis. Fifteen micrograms of mitochondrial proteins from mutant and WT zebrafish were electrophoresed through a Blue-Native gel, electroblotted and hybridized with antibodies for Ndufs1, Uqcrc2, Cox5a, Atp5c (subunits of complex I, III, IV and V, respectively) as well as Sdha (subunit of complex II) as a loading control. (B) Quantification of levels of complexes I, III, IV and V in mutant and WT zebrafish. (C) The activities of OXPHOS complexes were investigated by enzymatic assays on complexes I, II, III, IV and V in mitochondria isolated from mutant and WT zebrafish. The calculations were based on three independent determinations. Graph details and symbols are explained in the legend to Figure 2.

Expression Data

Gene:	sdha
Antibodies:	Ab1-atp5c1 Ab1-cox5a Ab1-ndufs1 Ab2-uqcrc2
Fish:	AB mto1^{zf3385/zf3385} (AB) mto1^zf3385/+ (AB)
Anatomical Term:	whole organism
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mto1^{zf3385/zf3385} (AB) mto1^zf3385/+ (AB)
Observed In:	cytochrome-c oxidase activity mitochondrial electron transport, NADH to ubiquinone mitochondrial electron transport, ubiquinol to cytochrome c whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

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