IMAGE

Figure 6.

ID

ZDB-IMAGE-210512-33

Genes

sdha

Antibodies

Source

Figures for Zhang et al., 2021

Image

Figure Caption

Figure 6.

The instability of OXPHOS complexes. (A) The steady-state levels of five OXPHOS complexes by Blue-Native gel electrophoresis. Fifteen micrograms of mitochondrial proteins from mutant and WT zebrafish were electrophoresed through a Blue-Native gel, electroblotted and hybridized with antibodies for Ndufs1, Uqcrc2, Cox5a, Atp5c (subunits of complex I, III, IV and V, respectively) as well as Sdha (subunit of complex II) as a loading control. (B) Quantification of levels of complexes I, III, IV and V in mutant and WT zebrafish. (C) The activities of OXPHOS complexes were investigated by enzymatic assays on complexes I, II, III, IV and V in mitochondria isolated from mutant and WT zebrafish. The calculations were based on three independent determinations. Graph details and symbols are explained in the legend to Figure 2.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.