FIGURE

Figure 4.

ID

ZDB-FIG-210512-31

Publication

Zhang et al., 2021 - Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency

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Figure 4.

Analysis of mitochondrial tRNA conformation. (A) Northern blot analysis of tRNAs under native condition. Five micrograms of total RNAs from mutant and WT zebrafish were electrophoresed through a native polyacrylamide gel, electroblotted and hybridized with the DIG-labeled oligonucleotide probes as tRNA^Gln, tRNA^Trp, tRNA^Leu(UUR), tRNA^Lys and tRNA^Ala, respectively. (B) S1 digestion patterns of tRNA^Gln, tRNA^Trp, tRNA^Leu(UUR), tRNA^Lys and tRNA^Ala. Two micrograms of RNAs from mto1^−/− and WT zebrafish were used for the S1 cleavage reaction at various lengths (from 0 to 120 min). Cleavage products of tRNAs were resolved in 10% denaturating PAGE gels with 8 M urea, electroblotted and hybridized with 3′ end DIG-labeled oligonucleotide probes specific for tRNA^Gln, tRNA^Trp, tRNA^Leu(UUR), tRNA^Lys and tRNA^Ala, respectively. Arrows denoted the 30–40 nt regions showing 3′ tRNA half.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mto1^{zf3385/zf3385} (AB)
Observed In:	whole organism tRNA(Gly) whole organism tRNA(Leu) whole organism tRNA(Lys) whole organism tRNA(Trp)
Stage:	Adult

Phenotype Detail

Acknowledgments

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