ZFIN Image: Zhang et al., 2021, Figure 4.

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Figure Caption

Figure 4.

Analysis of mitochondrial tRNA conformation. (A) Northern blot analysis of tRNAs under native condition. Five micrograms of total RNAs from mutant and WT zebrafish were electrophoresed through a native polyacrylamide gel, electroblotted and hybridized with the DIG-labeled oligonucleotide probes as tRNA^Gln, tRNA^Trp, tRNA^Leu(UUR), tRNA^Lys and tRNA^Ala, respectively. (B) S1 digestion patterns of tRNA^Gln, tRNA^Trp, tRNA^Leu(UUR), tRNA^Lys and tRNA^Ala. Two micrograms of RNAs from mto1^−/− and WT zebrafish were used for the S1 cleavage reaction at various lengths (from 0 to 120 min). Cleavage products of tRNAs were resolved in 10% denaturating PAGE gels with 8 M urea, electroblotted and hybridized with 3′ end DIG-labeled oligonucleotide probes specific for tRNA^Gln, tRNA^Trp, tRNA^Leu(UUR), tRNA^Lys and tRNA^Ala, respectively. Arrows denoted the 30–40 nt regions showing 3′ tRNA half.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.