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TRAP1 is induced following hypoxia.a Western blot analysis (left) and protein quantification (right) of TRAP1 expression level in embryos exposed to hypoxia (5% O2) from the stage of 48 hpf for 24 and 48 h. b TRAP1 expression profile was analyzed at 96 hpf following short-term hypoxia treatment as indicated. c Western blot analysis of TRAP1 expression in Zebrafish at 5 dpf following short-term hypoxia treatment. d Western blot analysis and quantification of TRAP1 expression in MIA PaCa-2 human pancreatic adenocarcinoma cells following 2–6 h of hypoxia (0.5% O2) or treatment with CoCl2 (0.5 mM for 6 h). The mitochondrial protein citrate synthase and actin were used as loading controls for fish and human cells, respectively. Data are reported as average ±SEM of at least three independent experiments with an unpaired two-tailed Student’s t test; asterisks indicate significant differences (*p < 0.05, **p < 0.01, ***p < 0.001).
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