FIGURE

Fig. 5

ID

ZDB-FIG-210508-15

Publication

Laquatra et al., 2021 - HIF1α-dependent induction of the mitochondrial chaperone TRAP1 regulates bioenergetic adaptations to hypoxia

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Fig. 5

TRAP1 is overexpressed in pancreatic tumors and downregulates SDH activity.

a Western blot analysis and protein quantification of TRAP1 in wild-type pancreas and in pancreatic tumors driven by KRAS^G12D expression (1 year post-fertilization, ypf). Values were normalized for citrate synthase. b Analysis of TRAP1 mRNA expression in wild-type pancreas and in pancreatic tumors at 1 ypf. Values were normalized for expression of rpl13 and actin, used as housekeeping genes. The number of animals used in each condition is reported inside column bars. c Colorimetric assay (pink staining) of SDH activity in tissue slides of wild-type pancreas and pancreatic tumors with/without the TRAP1 specific inhibitor compound 5 (100 μM, 30 min pre-incubation). In (a, b), data are reported as average ±SEM of at least four different animals per condition with an unpaired two-tailed Student’s t test; asterisks indicate significant differences (*p < 0.05).

Expression Data

Gene:	trap1
Fish:	TU jh16Tg; mk202Tg (TU)
Anatomical Term:	pancreas
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	jh16Tg; mk202Tg (TU)
Observed In:	pancreas
Stage:	Adult

Phenotype Detail

Acknowledgments

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