Figure 2—figure supplement 1.

MZ<italic>rab25a</italic> and MZ<italic>rab25b</italic> phenotypic characterization.

(A) qPCR analysis of rab25a, rab25b, and rab11a transcripts levels in WT, MZrab25a, and MZrab25b embryos at shield stage. (B) Time-matched phalloidin stained WT and MZrab25b double mutants at 8hpf. Scale bar 100 μm. (C) Tg: MZrab25b (ß-actin;rab25b) epiboly movements rescued compared to MZrab25b mutants. WT (n = 8), MZrab25b (n = 6), and MZrab25b Tg (ß-actin; rab25b) (n = 7) time-matched at 8hpf. Means:SEM; One-Way ANOVA, *p<0.05. (D) Bright-field images of WT and MZrab25b embryos positioned laterally at 3hpf. (E) Stage-matched WT, MZrab25a and MZrab25b embryos at 75% epiboly showing phalloidin staining of F-actin (red) and sytox green labeling of nuclei (green). Embryos positioned laterally with animal pole to the top; Scale bar 100 μm. (F) Whole-mount in situ hybridizations using indicated probes at indicated stages. Scale bar 100 μm. Arrows indicate sox17 staining.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to 75%-epiboly

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: 1k-cell to 75%-epiboly

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife