FIGURE

Figure 3.

ID

ZDB-FIG-210414-55

Publication

Willoughby et al., 2021 - The recycling endosome protein Rab25 coordinates collective cell movements in the zebrafish surface epithelium

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Figure 3.

Unstable cytokinetic bridges and delayed abscission in MZ<italic>rab25b</italic> embryos.

(A) Confocal z-projections of stage-matched WT and MZrab25b embryos at 50% epiboly expressing mGfp (green) and H2A-Rfp (magenta). Embryos positioned laterally. Scale bar 40 μm. (B) Apical surface area quantifications of WT mononucleate (n = 151, N = 6), MZrab25a binucleate (n = 10, N=6) and MZrab25b bi-(n = 3, N=4) and multinucleate (n = 9, N=4) at 80% epiboly. Mean: SEM; One-Way ANOVA; ****, p<0.0001. (C–C”) z-projection of stills from confocal time-lapses of WT and MZrab25b embryos labeled for microtubules (green), nuclei (magenta), and plasma membrane (magenta) starting at dome stage; white arrow marks WT bridge; yellow arrows marks bridge regression in MZrab25b cell. (C”) Red arrowheads indicate cytokinetic bridge torn open by neighboring morphogenic stress. Scale bar 20 μm. (D) Intercellular bridge duration in WT (n = 17, N = 3) and MZrab25b (n = 15, N = 4) embryos during epiboly initiation in EVL marginal regions. Mean: SEM; Mann- Whitney Test; ***, p<0.001. (E) Intercellular bridge length following formation in WT (n = 17,N = 3) and MZrab25b (n = 26,N = 4) embryos during epiboly initiation in EVL marginal regions. Mean: SEM; Mann-Whitney Test; *p<0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rab25b^uot11/uot11
Observed In:	EVL abscission EVL cytokinesis EVL intercellular bridge EVL intercellular bridge organization
Stage:	Dome

Phenotype Detail

Acknowledgments

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