FIGURE

Figure 1

ID

ZDB-FIG-210301-74

Publication

Brastrom et al., 2021 - Functional Role of the RNA-Binding Protein Rbm24a and Its Target sox2 in Microphthalmia

Other Figures

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Figure 1

Mutation of rbm24a phenocopies morpholino knockdown. (A) Schematic for genetic structure of Rbm24a, with the RNA recognition motif (RRM) in blue and the Ala-rich region in green. The red line indicates the site of the Alt-R CRISPR/Cas0 mutation. (B) Uninjected control 4 dpf embryo with wild type morphology. (B’) Detail of eye shown in B. (C) and (D) are Alt-R CRISPR/Cas9-injected embryos showing the variation of the phenotypes. (C’) and (D’) are detail of eyes found in C and D, respectively. (E) Graph of uninjected and CRISPR-injected F0 embryos. (F) Synthego ICE analysis of mutations found in F0 mutants. Indels are listed. Images taken at 33×.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR4-rbm24a
Knockdown Reagent:	CRISPR4-rbm24a
Observed In:	eye heart
Stage:	Day 4

Phenotype Detail

Acknowledgments

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