PUBLICATION
Functional Role of the RNA-Binding Protein Rbm24a and Its Target sox2 in Microphthalmia
- Authors
- Brastrom, L.K., Scott, C.A., Wang, K., Slusarski, D.C.
- ID
- ZDB-PUB-210128-1
- Date
- 2021
- Source
- Biomedicines 9(2): (Journal)
- Registered Authors
- Slusarski, Diane C.
- Keywords
- RNA binding protein, microphthalmia, post-transcriptional regulation, rbm24a, sox2, vision, visual assay, zebrafish
- MeSH Terms
- none
- PubMed
- 33494192 Full text @ Biomedicines
Citation
Brastrom, L.K., Scott, C.A., Wang, K., Slusarski, D.C. (2021) Functional Role of the RNA-Binding Protein Rbm24a and Its Target sox2 in Microphthalmia. Biomedicines. 9(2):.
Abstract
Congenital eye defects represent a large class of disorders affecting roughly 21 million children worldwide. Microphthalmia and anophthalmia are relatively common congenital defects, with approximately 20% of human cases caused by mutations in SOX2. Recently, we identified the RNA-binding motif protein 24a (Rbm24a) which binds to and regulates sox2 in zebrafish and mice. Here we show that morpholino knockdown of rbm24a leads to microphthalmia and visual impairment. By utilizing sequential injections, we demonstrate that addition of exogenous sox2 RNA to rbm24a-deplete embryos is sufficient to suppress morphological and visual defects. This research demonstrates a critical role for understanding the post-transcriptional regulation of genes needed for development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping