FIGURE

Figure 2.

ID

ZDB-FIG-200829-65

Publication

Liu et al., 2020 - TMEM216 Deletion Causes Mislocalization of Cone Opsin and Rhodopsin and Photoreceptor Degeneration in Zebrafish

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Figure 2.

Two tmem216 knockout zebrafish lines were generated by genome editing. Zebrafish knockout lines, tmem216^snyΔ175 and tmem216^snyR8Δ60, were generated by CRISPR/Cas9 genome editing. (A) Alignment of mutant genomic sequences with wildtype sequence from exon 3 to exon 4. The tmem216^snyΔ175 line features two deletions of two stretches of DNA of 172 and three nucleotides. The tmem216^snyR8Δ60 line features an eight-nucleotide repeat and deletion of two stretches of DNA of 56 and four nucleotides. Both mutations were expected to result in frameshift. (B) Protein sequence alignment between wildtype TMEM216, and the expected TMEM216^SNYΔ175 and TMEM216^SNYR8Δ60 mutant proteins. Note both mutations result in the loss of all four transmembrane domains. (C) RT-PCR of wild-type, heterozygous, and homozygous zebrafish, showing that homozygous knockouts express only mutant mRNA.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.