FIGURE

Figure 1

ID

ZDB-FIG-200805-7

Publication

Grivas et al., 2020 - Loss of Caveolin-1 and caveolae leads to increased cardiac cell stiffness and functional decline of the adult zebrafish heart

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Figure 1

Caveolin-1 is expressed in the endothelium, endocardium and epicardium of the intact and injured adult zebrafish heart. (a) Immunofluorescence staining of Cav1 and Tropomyosin (cardiomyocytes) in an intact Tg(fli1a:GFP) heart. ba, bulbus arteriosus; v, valves. (b–c′′) Cav1 immunoreactivity in the epicardium (arrows) overlaps with GFP in the endothelium (asterisks) and endocardium (arrowheads). Cav1 is also expressed in the zone between the cortical and trabecular cardiomyocytes (insert in b′). (d–f′) Cav1 immunostaining in 7 dpci Tg(wt1b:GFP) heart. The dashed area in (d) marks the injured area; Cav1 is expressed in the activated epicardium (e–f′ brackets) and endocardium (f, arrows) upon injury. (g–h′) Immunolabelling of Cav1 in a 7 dpci Tg(fli1a:GFP) heart. (h–h′) Arrows indicate Cav1⁺ endocardial cells. Scale bars: 100 μm in (a), (d), (e) and 50 μm in other panels. (i) qPCR analysis of caveolae-related genes during regeneration. Mean ± s.d., Brown-Forsythe and Welch ANOVA tests, *P < 0.05, **P < 0.01.

Expression Data

Genes:	cav1 cav2 cav3 cavin1b
Antibody:	Ab1-cav1
Fish:	WT li1Tg y1Tg
Condition:	cryoablation: cardiac ventricle
Anatomical Terms:	bulbus arteriosus endocardium epicardium heart valve heart vasculature regenerating tissue endocardium regenerating tissue epicardium whole organism
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	cryoablation: cardiac ventricle
Observed In:	whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

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