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RA regulates her9 expression but Her9 is not required for the effects of RA on opsin expression. WISH for her9 expression at 36 hpf in control treated (A), DEAB treated (A′) and RA treated (A″) retinas. (B) qPCR for her9 expression in heads of 36 hpf zebrafish embryos following drug treatments. N = 20 heads per biological replicate, 3 biological replicates per drug treatment. *p < 0.05. GFP expression in control treated versus RA-treated XOPs:GFP; her9+/+ (C–C′″) or XOPs:GFP; her9−/− (D–D′″) retinas. (E) Cell counts comparing rhodopsin expressing cells in the untreated and treated retinas. (F–F′″) IHC for UV opsin in control versus RA-treated TαC:GFP; her9+/+ (F–F′″) or TαC:GFP; her9−/− (G–G′″) retinas. (H) Cell counts comparing UV opsin expressing cells in the untreated and treated retinas (# of opsin+/100 µm). CF, choroid fissure; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; L, lens; Scale bar = 50 µm.
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