FIGURE

Figure 1

ID

ZDB-FIG-200621-1

Publication

Zou et al., 2020 - Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription

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Figure 1

Emodin increases SerRS expression and suppresses VEGFA expression in MDA-MB-231 cells. (A) Diagram illustrating system for screening in house library of compounds from Chinese herbs for increased SerRS expression (TF, transcriptional factor). (B) Heatmap of 330 compounds that regulate SerRS expression based on firefly/renilla ratio. Compared with DMSO, compounds with >1.0-fold change were regarded as effective compounds. (C) Chemical structure of emodin. (D) Effect of concentration of emodin and duration of treatment on firefly/renilla ratio. (E) mRNA level of SerRS from MDA-MB-231 cells; effect of concentration and treatment time with emodin. (F) Protein level of SerRS from MDA-MB-231 cells treated with emodin (10 µM) for 48 h. (G) mRNA level of VEGFA from MDA-MB-231 cells; effect of concentration and treatment time with emodin. (H) Protein level of VEGFA from MDA-MB-231 cells treated with emodin for 48 h. (I) ELISA detection of VEGFA expression in MDA-MB-231 cells treated with emodin for 48 h. (J) mRNA level of SerRS in MDA-MB-231 cells following lentiviral infection of shRNA targeting human SerRS. (K) mRNA levels of VEGFA from MDA-MB-231-shLacZ and MDA-MB-231-shSerRS cells treated with emodin for 48 h. n=3 for D-K; data are means ± SD., *p<0.05; **p<0.01; ***p<0.001; ns, no significant difference; by one-way ANOVA for D, E, G; by Student's t-test for I-K.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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