FIGURE

Fig. S1

ID

ZDB-FIG-191024-6

Publication

Djannatian et al., 2019 - Two adhesive systems cooperatively regulate axon ensheathment and myelin growth in the CNS

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

a-d Co-expression of UAS-driven fusion proteins with HuC:Gal4 (for neuronal expression) or Sox10:Gal4 (for glial expression) reveals nodal expression of EGFP-Cntn1a (a, 4 dpf) and paranodal expression of EGFP-Cntn1b (b, 7 dpf), Caspr-YFP (c, 5 dpf) and Nfascb-EGFP (d, 3 3

dpf) in Tg(Sox10:mRFP) dorsal spinal cord axons. e Phenotypes of adult wild-type (wt) and mutant zebrafish (90 dpf). Scale bar ticks represent 0.5 cm. f-i Validation of zebrafish mutant lines by quantitative PCR using the Ct method. Graphs represent fold expression change of mutant fish compared to age-matched wt fish. cntn1b-/- del2 (f, n = 3 fish, unpaired two sided t test), caspr-/- del1 (g, n = 3, unpaired two sided t test), nfascb-/- del10 (h, n = 3, unpaired two sided t test), mag-/- del2/ins3 and mag-/- del18/ins7 (i , n = 3, one-way ANOVA, p < 0.0001). Bonferroni-corrected p values: ***p < 0.001. Data are presented as means ± s.d. Scale bars, 2 m. Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.