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Fig. S1
a-d Co-expression of UAS-driven fusion proteins with HuC:Gal4 (for neuronal expression) or Sox10:Gal4 (for glial expression) reveals nodal expression of EGFP-Cntn1a (a, 4 dpf) and paranodal expression of EGFP-Cntn1b (b, 7 dpf), Caspr-YFP (c, 5 dpf) and Nfascb-EGFP (d, 3 3
dpf) in Tg(Sox10:mRFP) dorsal spinal cord axons. e Phenotypes of adult wild-type (wt) and mutant zebrafish (90 dpf). Scale bar ticks represent 0.5 cm. f-i Validation of zebrafish mutant lines by quantitative PCR using the Ct method. Graphs represent fold expression change of mutant fish compared to age-matched wt fish. cntn1b-/- del2 (f, n = 3 fish, unpaired two sided t test), caspr-/- del1 (g, n = 3, unpaired two sided t test), nfascb-/- del10 (h, n = 3, unpaired two sided t test), mag-/- del2/ins3 and mag-/- del18/ins7 (i , n = 3, one-way ANOVA, p < 0.0001). Bonferroni-corrected p values: ***p < 0.001. Data are presented as means ± s.d. Scale bars, 2 m. Source data are provided as a Source Data file.