FIGURE

Fig. 1

ID

ZDB-FIG-191024-2

Publication

Djannatian et al., 2019 - Two adhesive systems cooperatively regulate axon ensheathment and myelin growth in the CNS

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Fig. 1

Loss of adhesion molecules of the paranodal axo-glial junction results in neuronal cell body wrapping. a CNS myelin of 3, 4 and 10 dpf wild-type (wt) and caspr^−/− fish. Note cell body wrappings in caspr^−/− fish. b Cell body wrappings per myelinated area at 3, 4, and 10/11 dpf ( n = 6–10, Kruskal Wallis ANOVA: 3 dpf: p = 0.0004, 4 dpf and 10/11 dpf: p < 0.0001). c Cell body wrappings in wt fish ( n = 8–9). Data derived from panel ( b). Mann-Whitney test, * p = 0.0399. d HuC:mCherry expression in 4 dpf caspr^−/− mag^−/− fish shows neuronal cell bodies enwrapped by myelin membrane. e SEM images of cell body wrappings in cntn1b^−/− fish at dpf 10. f Sheath retractions (arrow) from neuronal cell bodies (30 min intervals, 15–16 h time lapse experiments, n = 3, Kruskal-Wallis one-way ANOVA: p = 0.4393). Images ( a, d, f) are maximum intensity projections of Tg(mbp:EGFP-CAAX) zebrafish dorsal spinal cord. p values, * < 0.05, ** < 0.01, *** < 0.001. Data are presented as means ± s.d.Scale bars, 5 mm ( a, d, f), 2 µm ( e). Source data are provided as a Source Data file

Expression Data

Gene:	EGFP
Fish:	ue2Tg cntnap1^{tum205/tum205}; ue2Tg
Anatomical Term:	spinal cord myelin sheath
Stage Range:	Protruding-mouth to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cntn1b^{tum204/tum204}; ue2Tg cntnap1^{tum205/tum205}; ue2Tg mag^{tum202/tum202}; ue2Tg nfascb^{tum203/tum203}; ue2Tg
Observed In:	spinal cord central nervous system myelin formation spinal cord myelin sheath spinal cord neuronal cell body
Stage Range:	Protruding-mouth to Days 7-13

Phenotype Detail

Acknowledgments

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