FIGURE

FIGURE 5

ID

ZDB-FIG-190723-168

Publication

Waldron et al., 2019 - Knock-Down of Histidyl-tRNA Synthetase Causes Cell Cycle Arrest and Apoptosis of Neuronal Progenitor Cells in vivo

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 5

The amino acid starvation response (AASR) is activated in hars KD embryos and results in G1-arrest of retinal progenitor cells. (A–F’) In situ hybridization for three AASR genes in 48 hpf hars KD embryos compared to controls. (G,H) Semi-quantitative RT-PCR for the AASR genes. (H) Quantification of AASR gene expression relative to ef1α in control and hars KD embryos. (I) Schematic showing the relationship between AASR and cell cycle regulation. (J) Results of DNA content analysis on GFP+ cells from 18 hpf (Tg(Rx3:GFP)) uninjected and hars KD embryos. Percentages shown in table are the mean percentages of three replicates. Student’s t-test (n = 3 ^∗ = 0.05, ^∗∗= 0.01).

Expression Data

Genes:	asns eif4ebp1 gpt2
Fish:	TL + MO1-hars
Knockdown Reagent:	MO1-hars
Anatomical Terms:	head whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TL + MO1-hars
Knockdown Reagent:	MO1-hars
Observed In:	head whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol