|
FIGURE 5
The amino acid starvation response (AASR) is activated in hars KD embryos and results in G1-arrest of retinal progenitor cells. (A–F’) In situ hybridization for three AASR genes in 48 hpf hars KD embryos compared to controls. (G,H) Semi-quantitative RT-PCR for the AASR genes. (H) Quantification of AASR gene expression relative to ef1α in control and hars KD embryos. (I) Schematic showing the relationship between AASR and cell cycle regulation. (J) Results of DNA content analysis on GFP+ cells from 18 hpf (Tg(Rx3:GFP)) uninjected and hars KD embryos. Percentages shown in table are the mean percentages of three replicates. Student’s t-test (n = 3 ∗ = 0.05, ∗∗= 0.01).