FIGURE

Fig. S1

ID

ZDB-FIG-181107-5

Publication

Wang et al., 2018 - Anosmin1 Shuttles Fgf to Facilitate Its Diffusion, Increase Its Local Concentration, and Induce Sensory Organs

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Fig. S1

Fgf ligand expression in wild-type and atoh1a MO-injected embryos, Related to Figure 1.

(A) Expression of fgf3, fgf10a, fgfr1a, and fgfr1b mRNA (purple) at 32 hpf. Arrows indicate expression in neuromasts and arrowhead denotes the position of the primordium. Scale bar = 500 μm.

(B) fgf10a mRNA expression at 32 hpf in a control embryo (left) and an embryo injected with a morpholino against atoh1a (right). Arrows indicate fgf10a expression in central cells of deposited neuromasts, and arrowheads indicate fgf10a expression in the front of the primordium. Scale bar = 500 μm.

(C) Maximum intensity projection of neuromast 1 in embryos of the indicated genotypes. Left, composite of GFP from cldnb:lyn2GFP (green) and fgf10a mRNA (false-colored red). Middle, GFP only. Right, fgf10a mRNA only. Scale bar = 25 μm.

(D) Maximum intensity projection of the primordium in live, heat-shocked embryos of the indicated genotypes. Top, composite of GFP from cldnb:lyn2GFP (green) and secreted mCherry from hs:sec-mCherry (red). Bottom, mCherry only. Scale bar = 50 μm.

Expression Data

Genes:	EGFP fgf3 fgf10a fgfr1a fgfr1b mCherry
Fish:	WT WT + MO4-atoh1a sk87Tg; zf106Tg fgf3^t24149/+; zf106Tg fgf3^{t24149/t24149}; zf106Tg fgf3^t24149/+; zf106Tg + MO4-atoh1a fgf3^{t24149/t24149}; zf106Tg + MO4-atoh1a fgf10a^tbvbo/tbvbo; fgf3^{t24149/t24149}; sk87Tg; zf106Tg
Knockdown Reagent:	MO4-atoh1a
Anatomical Terms:	neuromast posterior lateral line primordium
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fgf3^t24149/+; zf106Tg + MO4-atoh1a fgf3^{t24149/t24149}; zf106Tg + MO4-atoh1a fgf10a^tbvbo/tbvbo; fgf3^{t24149/t24149}; sk87Tg; zf106Tg
Knockdown Reagent:	MO4-atoh1a
Observed In:	neuromast
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 46(6), Wang, J., Yin, Y., Lau, S., Sankaran, J., Rothenberg, E., Wohland, T., Meier-Schellersheim, M., Knaut, H., Anosmin1 Shuttles Fgf to Facilitate Its Diffusion, Increase Its Local Concentration, and Induce Sensory Organs, 751-766.e12, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell