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Fig. 1

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ZDB-FIG-181106-2

Publication

Wang et al., 2018 - Anosmin1 Shuttles Fgf to Facilitate Its Diffusion, Increase Its Local Concentration, and Induce Sensory Organs

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Fig. 1

FGF Ligand and Receptor Expression and Function in Sensory Organ Formation

(A) fgf3, fgf10a, fgfr1a, and fgfr1b mRNA expression (red) in the primordium (green) at 32 hpf. Scale bar represents 100 μm.

(B) Primordium position in embryos of indicated genotypes at 50 hpf. White arrows denote deposited neuromasts, arrowheads denote position of primordium, and red arrows indicate position of primordium at the beginning of the heat shock. Scale bar represents 500 μm.

(C) Plot of the number of neuromasts for (E) and (B). Mean, SD, and individual data points shown. ^∗∗ = p < 0.01, ^∗∗∗∗ = p < 0.0001, ns = not significant. ANOVA p < 0.0001.

(D) Quantification of the number of neuromasts deposited after heat shock in embryos of the indicated genotypes. Mean, SD, and individual data points shown. ^∗∗ = p < 0.01, ^∗∗∗ = p < 0.001. ANOVA p < 0.0001.

(E) Neuromast deposition (arrows) and primordium migration (arrowhead) in the embryos of indicated genotype at 50 hpf. Scale bar represents 500 μm.

(F) Table of p values calculated with Sidak's multiple comparisons test with a single pooled variance for the pairwise comparisons shown. ANOVA p < 0.0001.

See also Figure S1 and Video S1.

Expression Data

Genes:	EGFP fgf3 fgf10a fgfr1a fgfr1b mCherry
Fish:	zf106Tg zf106Tg + MO4-atoh1a fgf3^{t24149/t24149}; zf106Tg fgfr1a^t2227/t2227; sk85Tg anos1a^sk50/+; anos1a^sk51/+; anos1b^sk52/+; anos1b^sk53/+; zf106Tg fgf3^{t24149/t24149}; zf106Tg + MO4-atoh1a fgf10a^tbvbo/tbvbo; fgf3^{t24149/t24149}; zf106Tg anos1a^sk50/+; anos1a^sk51/+; anos1b^sk52/+; anos1b^sk53/+; zf106Tg + MO4-atoh1a fgf3^{t24149/t24149}; anos1a^sk50/+; anos1a^sk51/+; anos1b^sk52/+; anos1b^sk53/+; zf106Tg fgf3^{t24149/t24149}; anos1a^sk50/+; anos1a^sk51/+; anos1b^sk52/+; anos1b^sk53/+; zf106Tg + MO4-atoh1a
Knockdown Reagent:	MO4-atoh1a
Anatomical Terms:	posterior lateral line neuromast posterior lateral line primordium
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fgfr1a^t2227/t2227 WT + MO4-atoh1a pd1Tg sk88Tg zf106Tg + MO4-atoh1a anos1a^sk50/+; anos1a^sk51/+; anos1b^sk52/+; anos1b^sk53/+ fgf3^{t24149/t24149}; zf106Tg + MO4-atoh1a anos1a^sk50/+; anos1a^sk51/+; anos1b^sk52/+; anos1b^sk53/+ + MO4-atoh1a anos1a^sk50/+; anos1a^sk51/+; anos1b^sk52/+; anos1b^sk53/+; zf106Tg + MO4-atoh1a fgf3^{t24149/t24149}; anos1a^sk50/+; anos1a^sk51/+; anos1b^sk52/+; anos1b^sk53/+ fgf3^{t24149/t24149}; anos1a^sk50/+; anos1a^sk51/+; anos1b^sk52/+; anos1b^sk53/+ + MO4-atoh1a fgf3^{t24149/t24149}; anos1a^sk50/+; anos1a^sk51/+; anos1b^sk52/+; anos1b^sk53/+; zf106Tg + MO4-atoh1a
Condition:	heat shock
Knockdown Reagent:	MO4-atoh1a
Observed In:	posterior lateral line neuromast
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 46(6), Wang, J., Yin, Y., Lau, S., Sankaran, J., Rothenberg, E., Wohland, T., Meier-Schellersheim, M., Knaut, H., Anosmin1 Shuttles Fgf to Facilitate Its Diffusion, Increase Its Local Concentration, and Induce Sensory Organs, 751-766.e12, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell