FIGURE

Fig. 2

ID

ZDB-FIG-180713-35

Publication

Yan et al., 2018 - Activation of Hepatic Stellate Cells During Liver Carcinogenesis Requires Fibrinogen/Integrin αvβ5 in Zebrafish

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Fig. 2

Fibrinogen production and integrin αvβ5 expression in the liver. (A) IF staining of Fibrinogen on liver sections of kras+ and WT larvae. Livers in the WT picture is marked by a dash line. (B) Quantification of Fibrinogen-positive hepatocytes. (C) Fold change of expression of fibrinogen genes (fga, fgb an fgg) kras^v12-expressing hepatocytes compared to WT hepatocytes. Integrin receptor, (D-E)expression of itgav (D) and itgb5 (E) mRNAs in hepatocytes and HSCs. Normal hepatocytes and HSCs were sorted by FACS based on DsRed and GFP expression from fabp10+ and hand2+ transgenic zebrafish respectively. Relative expression levels are shown with the value from hepatocytes arbitrarily set as 1. *P < .05. Scale bar: 20 μm.

Expression Data

Genes:	fga fgb fgg itgav itgb5
Antibody:	Ab1-fg
Fish:	gz32Tg gz15Tg; gz32Tg
Condition:	chemical treatment by environment: doxycycline
Anatomical Terms:	hematopoietic stem cell hepatocyte
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gz32Tg gz15Tg; gz32Tg
Condition:	chemical treatment by environment: doxycycline
Observed In:	hematopoietic stem cell hepatocyte
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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