FIGURE

Fig. 3

ID

ZDB-FIG-180411-7

Publication

Veil et al., 2017 - Maternal Nanog is critical for the zebrafish embryo architecture and for cell viability during gastrulation

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Fig. 3

Expression of mxtx2, eomesa and endomesodermal markers in Nanog mutants. Expression of the indicated genes in wild-type (WT), Mnanog and MZnanog embryos was visualized by in situ hybridization. Dorsal is to the right. (A) mxtx2 expression is absent in MZnanog and Mnanog (lateral view). (B) Sphere stage expression of sox32, mixer and eomesa (lateral view) and ntl (animal view). Note that sox32 and mixer are not detectable in MZnanog, but ntl and eomesa expression is not altered. (C) 30% epiboly stage expression for sox32, mixer, gata5 (lateral view) and foxD3 (animal view). f.c., forerunner cells (arrowhead). (D) sox17 and sox32 staining at 8 hpf in WT, MZnanog and MZspg (dorsal view). Note that sox17 and sox32 are both expressed in MZnanog, but only sox32 is expressed in MZspg. (E) sox17 staining in WT, MZnanog and Mnanog (9 hpf, lateral view). In D,E, arrows show scattered endodermal cells. (F) ntl expression at 6 hpf (lateral view). (G) eomesa expression at 5.3 hpf (lateral view). Scale bars: 100 µm.

Expression Data

Genes:	eomesa foxd3 gata5 mixl1 mxtx2 sox17 sox32 tbxta
Fish:	WT nanog^m1435/m1435 pou5f3^m793/m793 nanog^m1435
Anatomical Terms:	endoderm margin presumptive endoderm presumptive mesoderm whole organism yolk syncytial layer
Stage Range:	30%-epiboly to 90%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nanog^m1435/m1435 pou5f3^m793/m793 nanog^m1435
Observed In:	endoderm presumptive endoderm presumptive mesoderm whole organism yolk syncytial layer
Stage Range:	Sphere to 90%-epiboly

Phenotype Detail

Acknowledgments

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