FIGURE

Fig. S5

ID

ZDB-FIG-180129-17

Publication

Giger et al., 2017 - Endodermal germ-layer formation through active actin-driven migration triggered by N-cadherin

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Fig. S5

Creation of mosaic embryos by plasmid DNA injection. (A) Injection of plasmid DNA at the one-cell stage leads to mosaic expression. (B–G) Optical sections of mosaic embryos created by plasmid DNA injection of either Lifeact-GFP (B and D) or Tar*:HSE:Lifeact-GFP (C and E). Cells emit large cytoplasmic extensions (B and C) oriented toward the YSL (D and E) at the same frequency (F) and of the same length (G) as with cell transplantation. n_ecto _transplant = 71 extensions, n_ecto _DNA = 54 extensions, P_angle _ecto _{transplant/DNA} = 0.55; n_endo _transplant = 72 extensions, n_endo _DNA = 46 extensions, P_angle _endo _{transplant/DNA} = 0.46. ns, nonsignificant (P > 0.05). (H–L) Distribution of embryos according to the percentage of internalized cells at midgastrulation plotted as histograms (H–K) or as a cumulative plot (L). ***P < 0.001. (Scale bar: 10 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA