FIGURE

Fig. 1

ID

ZDB-FIG-180129-10

Publication

Giger et al., 2017 - Endodermal germ-layer formation through active actin-driven migration triggered by N-cadherin

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Fig. 1

Endodermal cells rapidly migrate to the YSL. (A) Lifeact-GFP–expressing endodermal cells were transplanted to the margin of embryos expressing membrane-bound mCherry, just beneath the EVL. 4D stacks were acquired, and sagittal sections were reconstructed. (B) Sagittal sections showing endodermal cell internalization (Movie S1). Arrowheads point to actin-rich cytoplasmic extensions. The dashed line delineates the surface of the embryo; the dotted line represents the limit between the blastoderm and the YSL. Animal pole is to the top. (C) Orientation of actin-rich protrusions. Dots represent measurements. (D) Movement of internalizing cells along the surface–YSL axis. The spatial and temporal origin is defined as the beginning of migration. (E) Instant speed of internalizing cells along the surface–YSL axis. Mean speed for each cell is represented as a colored segment. (F) Transplanted endodermal cells contribute to endodermal derivatives at 24 hpf (arrowhead). (Scale bar: 20 µm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA