Fig. S5

Fig. S5

Creation of mosaic embryos by plasmid DNA injection. (A) Injection of plasmid DNA at the one-cell stage leads to mosaic expression. (B–G) Optical sections of mosaic embryos created by plasmid DNA injection of either Lifeact-GFP (B and D) or Tar*:HSE:Lifeact-GFP (C and E). Cells emit large cytoplasmic extensions (B and C) oriented toward the YSL (D and E) at the same frequency (F) and of the same length (G) as with cell transplantation. n_{ecto transplant} = 71 extensions, n_{ecto DNA} = 54 extensions, P_{angle ecto transplant/DNA} = 0.55; n_{endo transplant} = 72 extensions, n_{endo DNA} = 46 extensions, P_{angle endo transplant/DNA} = 0.46. ns, nonsignificant (P > 0.05). (H–L) Distribution of embryos according to the percentage of internalized cells at midgastrulation plotted as histograms (H–K) or as a cumulative plot (L). ***P < 0.001. (Scale bar: 10 μm.)