FIGURE

Fig. 6

ID

ZDB-FIG-171113-51

Publication

Kotb et al., 2016 - Knockdown of ApoL1 in Zebrafish Larvae Affects the Glomerular Filtration Barrier and the Expression of Nephrin

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Fig. 6

zApoL1 KD reduces the expression of nephrin.

The expression of nephrin was significantly reduced in podocytes (green) after zApoL1 KD: zApoL1 TBM (A-D), zApoL1 SBM exon2 (E-H) and vivo-zApoL1 SBM exon2 (I-L) in contrast to CtrlMO (M-P) and untreated larvae (Q-T). RT-PCR shows a marked decrease of nephrin mRNA expression after zApoL1 knockdown compared to CtrlMO and control larvae. The elongation factor 1 (EF1) was used as a housekeeping gene. The right panel shows one representative experiment out of three. Scale bars represent 20 μm. (U) CtrlMO larvae have nicely developed foot processes in contrast to zApoL1 larvae that showed effacement of foot processes at some areas (V). Furthermore, the foot processes of zApoL1 KD larvae are sometimes surrounded by GBM (arrow in V) and developed microvilli-like structures protruding in the urinary space, respectively. Scale bar represents 1 μm.

Expression Data

Genes:	EGFP nphs1
Fish:	li4Tg li4Tg + MO1-apol1 li4Tg + MO3-apol1
Knockdown Reagents:	MO1-apol1 MO3-apol1
Anatomical Terms:	pronephric podocyte whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	li4Tg + MO1-apol1 li4Tg + MO3-apol1
Knockdown Reagents:	MO1-apol1 MO3-apol1
Observed In:	pronephric glomerulus morphogenesis pronephric podocyte pronephric podocyte podocyte foot whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

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