FIGURE

Fig. 4

ID

ZDB-FIG-171113-49

Publication

Kotb et al., 2016 - Knockdown of ApoL1 in Zebrafish Larvae Affects the Glomerular Filtration Barrier and the Expression of Nephrin

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Fig. 4

zApoL1 KD changes the morphology of the glomerulus.

zApoL1 was knocked down in larvae expressing EGFP in podocytes by zApoL1 TBM (A-D), zApoL1 SBM exon2 (E-H) and zApoL1 SBM exon6 (I-L) morpholinos. Paraffin sections of the larvae were stained with Alexa 546-phalloidin for F-actin and DAPI for the nuclei. The KD with three different morpholinos against zApoL1 resulted in a similar phenotype, comprising dilation of Bowman’s space (arrows in B, F, J), collapse and malformation of the glomerular tuft (asterisks in B, F, J). CtrlMO and Control larvae are shown in panel M-T. Scale bars represent 20 μm.

Expression Data

Gene:	EGFP
Fish:	li4Tg li4Tg + MO1-apol1 li4Tg + MO3-apol1 li4Tg + MO4-apol1
Knockdown Reagents:	MO1-apol1 MO3-apol1 MO4-apol1
Anatomical Term:	pronephric podocyte
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	li4Tg + MO1-apol1 li4Tg + MO3-apol1 li4Tg + MO4-apol1
Knockdown Reagents:	MO1-apol1 MO3-apol1 MO4-apol1
Observed In:	pronephric capsular space pronephric glomerular capillary pronephric glomerulus morphogenesis
Stage:	Day 4

Phenotype Detail

Acknowledgments

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