FIGURE

Fig. 6

ID

ZDB-FIG-171101-22

Publication

Felber et al., 2015 - Expression of osterix Is Regulated by FGF and Wnt/β-Catenin Signalling during Osteoblast Differentiation

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Fig. 6

FGF and Wnt/β-Catenin signalling interact to regulate osteoblast differentiation.

(A-D) Larvae were heat shocked at 48hpf, treated with SU5402 at 50hpf and fixed at 52hpf. Expression of osx is reduced after SU5402 (C) treatment and increased in hs:wnt8 larvae (B). Combined SU5402 and hs:wnt8 treatment still results in reduced expression of osx (D). (E-H) Larvae were heat shocked at 49hpf and fixed at 52hpf. Expression of osx is reduced in hs:dkk1 larvae (F) and increased in hs:fgf3 larvae (G). Expression is reduced in the combined treatment (H). (I-T) fgf3, sprouty1, sprouty4 and sef expression is inhibited by dkk1 over expression (K, N, Q, T) and activated by over expression of wnt8 (J, M, P, S). Arrowheads point to the opercle in the high magnification images to the right of each panel. Scale bars = 200μM.

Expression Data

Genes:	etv4 fgf3 il17rd sp7 spry1 spry4
Fish:	WT pd1Tg/+ w32Tg w34Tg zf115Tg w32Tg; zf115Tg
Conditions:	chemical treatment by environment: SU5402 heat shock
Anatomical Terms:	opercle pharyngeal arch 3-7
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT pd1Tg/+ w32Tg w34Tg zf115Tg w32Tg; zf115Tg
Conditions:	chemical treatment by environment: SU5402 heat shock
Observed In:	opercle pharyngeal arch 3-7
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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