FIGURE

Fig. 4

ID

ZDB-FIG-171101-21

Publication

Felber et al., 2015 - Expression of osterix Is Regulated by FGF and Wnt/β-Catenin Signalling during Osteoblast Differentiation

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Fig. 4

Wnt/β-Catenin signalling regulates osx expression.

(A-I) Larvae were treated at 48hpf and fixed 12hours later. Expression of runx2a (A-C) and runx2b (D-F) is unchanged after inhibition (hs:dkk1) or over activation (hs:wnt8) of Wnt/β-Catenin signalling. (G-L) Expression of osx is reduced in hs:dkk1 and increased in hs:wnt8 larvae 12 hours after the treatment. Arrowheads point to the opercle in the high magnification images to the right of each panel. Abbreviations: cl = cleithrum. Scale bar = 50μM. (J) Quantification for presence or absence of osx staining in the opercle from G and H. Expression was normalised to the control. (K) qPCR performed in parallel on g and h confirms the effects of Wnt/β-Catenin signalling on osx expression (p<0.01 using Student's t-test). Treatments were normalised to gapdh.

Expression Data

Genes:	runx2a runx2b sp7
Fish:	WT w32Tg w34Tg
Condition:	heat shock
Anatomical Term:	opercle
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	w32Tg
Condition:	heat shock
Observed In:	opercle
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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