Fig. 4
- ID
- ZDB-FIG-170703-17
- Publication
- Nelson et al., 2017 - In Vivo Regulation of the Zebrafish Endoderm Progenitor Niche by T-Box Transcription Factors
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Loss of Ta and Tbx16 Leads to Downregulation of Endodermal Specifiers and Reduction of Endodermal Progenitors during Gastrulation (A) qPCR analysis of mixl1, gata5, and sox32 at 50% epiboly (5.3 hpf) in single and double ta/tbx16 morphants. All genes are significantly downregulated in double morphants; ∗p ≤ 5 × 10−2; Student’s t test. Data are represented as mean ± SEM. See also Figure S2. (B) WISH analysis of sox32 in single and double morphants at germ ring stage (5.7 hpf). Arrowhead, YSL expression; ∗, loss of endoderm expression. (C) Microarray analysis at 90% epiboly (9 hpf) indicates downregulation of cxcl12a/b. Data are represented as mean ± SEM. (D) Immunological and WISH analysis of a sox17:eGFP transgene and endogenous sox17 expression at 90% epiboly (9 hpf) in single and double morphants. (E) Cell numbers identified by immunostaining and WISH in (D). Cell numbers are representative of at least 20 embryos per condition. ∗p ≤ 1 × 10−8; ∗∗p ≤ 1 × 10−20; Student’s t test. Data are represented as mean ± SEM. See also Figure S3. |
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| Stage Range: | 50%-epiboly to 90%-epiboly |
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| Stage Range: | 50%-epiboly to 90%-epiboly |