Fig. 4
- ID
- ZDB-FIG-170703-17
- Publication
- Nelson et al., 2017 - In Vivo Regulation of the Zebrafish Endoderm Progenitor Niche by T-Box Transcription Factors
- Other Figures
- All Figure Page
- Back to All Figure Page
Loss of Ta and Tbx16 Leads to Downregulation of Endodermal Specifiers and Reduction of Endodermal Progenitors during Gastrulation (A) qPCR analysis of mixl1, gata5, and sox32 at 50% epiboly (5.3 hpf) in single and double ta/tbx16 morphants. All genes are significantly downregulated in double morphants; ∗p ≤ 5 × 10−2; Student’s t test. Data are represented as mean ± SEM. See also Figure S2. (B) WISH analysis of sox32 in single and double morphants at germ ring stage (5.7 hpf). Arrowhead, YSL expression; ∗, loss of endoderm expression. (C) Microarray analysis at 90% epiboly (9 hpf) indicates downregulation of cxcl12a/b. Data are represented as mean ± SEM. (D) Immunological and WISH analysis of a sox17:eGFP transgene and endogenous sox17 expression at 90% epiboly (9 hpf) in single and double morphants. (E) Cell numbers identified by immunostaining and WISH in (D). Cell numbers are representative of at least 20 embryos per condition. ∗p ≤ 1 × 10−8; ∗∗p ≤ 1 × 10−20; Student’s t test. Data are represented as mean ± SEM. See also Figure S3. |
| Genes: | |
|---|---|
| Fish: | |
| Knockdown Reagents: | |
| Anatomical Terms: | |
| Stage Range: | Germ-ring to 90%-epiboly |
| Fish: | |
|---|---|
| Knockdown Reagents: | |
| Observed In: | |
| Stage Range: | 50%-epiboly to 90%-epiboly |