FIGURE

Fig. 2

ID

ZDB-FIG-170609-37

Publication

Theodore et al., 2017 - Distinct Roles for Matrix Metalloproteinases 2 and 9 in Embryonic Hematopoietic Stem Cell Emergence, Migration, and Niche Colonization

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Inhibition of Mmp2, but Not Mmp9, Function Affects EHT in the VDA

(A) Exposure to prinomastat (20 μM) or ARP-101 (10 μM; 12–36 hpf) caused abnormal runx1/cmyb patterning in the VDA, while MMP9-I (5 μM) had no effect.

(B) Qualitative phenotypic distribution of embryos from (A) scored with normal or abnormal runx1/cmyb expression (n ≥ 20/condition).

(C) In vivo imaging of Flk1:GFP at 36 hpf indicated that MMP inhibitor (12–36 hpf) treatment did not affect physical vasculature structure (n ≥ 5 embryos/condition).

(D) FACS analysis of double-positive HSPCs in Tg(kdrl:dsred/cmyb:gfp) embryos showed no difference in HSPCs after MMP inhibitor exposure (12–36 hpf; 5 embryos/sample, ≥3 replicates/condition).

(E) MO knockdown of mmp2 or mmp9 phenocopied effects of chemical inhibition on runx1/cmyb WISH at 36 hpf.

(F) Phenotypic distribution of embryos from (E) scored for runx1/cmyb expression (as in A) in the AGM (n value as in B).

(G) MO knockdown of mmp2 or mmp9 had no impact on Flk1:GFP⁺ endothelium (n value as in C).

(H) FACS analysis of Flk1:dsRed⁺/cMyb:GFP⁺ HSPCs showed no significant difference between mmp2 or mmp9 morphants and controls at 36 hpf (n value as in D).

Arrowheads mark HSPC clusters. Error bars denote mean ± SD. Scale bars, 100 μm.

Expression Data

Genes:	GFP myb runx1
Fish:	WT WT + MO1-mmp2 WT + MO4-mmp9 la116Tg la116Tg + MO1-mmp2 la116Tg + MO4-mmp9
Conditions:	chemical treatment by environment: N(2)-([biphenyl]-4-ylsulfonyl)-N-hydroxy-N(2)-isopropoxy-D-valinamide chemical treatment by environment: MMP9 inhibitor I chemical treatment by environment: prinomastat hydrochloride
Knockdown Reagents:	MO1-mmp2 MO4-mmp9
Anatomical Terms:	trunk vasculature ventral wall of dorsal aorta hematopoietic multipotent progenitor cell
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-mmp2 la116Tg la116Tg + MO1-mmp2 la116Tg + MO4-mmp9 pd27Tg; zf169Tg pd27Tg; zf169Tg + MO1-mmp2 pd27Tg; zf169Tg + MO4-mmp9
Conditions:	chemical treatment by environment: N(2)-([biphenyl]-4-ylsulfonyl)-N-hydroxy-N(2)-isopropoxy-D-valinamide chemical treatment by environment: MMP9 inhibitor I chemical treatment by environment: prinomastat hydrochloride
Knockdown Reagents:	MO1-mmp2 MO4-mmp9
Observed In:	hematopoietic multipotent progenitor cell trunk vasculature ventral wall of dorsal aorta hematopoietic multipotent progenitor cell
Stage:	Prim-25

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Stem Cell Reports