FIGURE

Fig. S5

ID

ZDB-FIG-170609-47

Publication

Theodore et al., 2017 - Distinct Roles for Matrix Metalloproteinases 2 and 9 in Embryonic Hematopoietic Stem Cell Emergence, Migration, and Niche Colonization

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Fig. S5

Mmp2 inhibition delays HSPC migration to the CHT without impacting circulation.

Related to Figure 5.

A) mmp2 mutants display inappropriate cmyb expression in the AGM at 72 hpf, phenocopying the effect of ARP- and MO-mediated Mmp2 inhibition.

B) Qualitative phenotypic distribution of total embryos from S5A (mmp2^+/- incross) scored with normal versus abnormal cmyb expression in the AGM at 72 hpf (n≥20 embryos/condition).

C) Embryos exposed to ARP (10μM, 12-48 hpf) showed reduced cmyb expression in the CHT, concurrent with enhanced expression in the AGM at 48 hpf, suggesting HSPCs are restrained in colonization of secondary hematopoietic sites with loss of Mmp2 function.

D) Qualitative phenotypic distribution of embryos from S5C scored with normal versus abnormal cmyb expression at 48 hpf (n≥20 embryos/condition).

E) In vivo imaging of Tg(kdrl:gfp/gata1a:dsred) embryos after exposure (12-72 hpf) to ARP, MMP9-I or Prinomastat showed no differences in Gata1⁺ erythrocyte circulation through the Flk1⁺ CHT vasculature compared to controls at 72 hpf (n≥10 embryos/condition).

Scale bars: 100μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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