FIGURE

Fig. 5

ID

ZDB-FIG-161116-13

Publication

Almeida et al., 2016 - Oligodendrocyte Development in the Absence of Their Target Axons In Vivo

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Fig. 5

pMN progenitor proliferation and motor neuron generation are unaffected by reduction in reticulospinal axons.

A, phospho-Histone 3 immunostained 50hpf Tg(olig2:EGFP) larvae. EGFP+ PH3+ cells indicated by arrowheads; 3A10 panels show labelled reticulospinal axons, or their absence, in corresponding area. Scale bar: 20μm. B, similar number of PH3+ EGFP+ cells between WT and mutants in 425μm long regions of the spinal cord 36–50 hpf. Data from N = 27 WT and N = 9 mutants (36hpf) and N = 15 WT and N = 10 mutants (50hpf). C, HB9 immunostained 50hpf larvae to label motor neurons. Scale bar: 12.5μm. D, similar number of motor neurons between WT and mutant in 150μm long regions of the spinal cord. Data from N = 13 WT and N = 9 mutants. Error bars indicate ± SD.

Expression Data

Gene:	EGFP
Antibodies:	Ab-3A10 Ab1-mnx1 Ab18-h3
Fish:	WT vu12Tg kifbp^st23/st23; vu12Tg
Anatomical Terms:	motor neuron reticulospinal neuron axon spinal cord axon spinal cord oligodendrocyte
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kifbp^st23/st23; vu12Tg
Observed In:	reticulospinal neuron axon spinal cord posterior region
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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