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Fig. 5
pMN progenitor proliferation and motor neuron generation are unaffected by reduction in reticulospinal axons.
A, phospho-Histone 3 immunostained 50hpf Tg(olig2:EGFP) larvae. EGFP+ PH3+ cells indicated by arrowheads; 3A10 panels show labelled reticulospinal axons, or their absence, in corresponding area. Scale bar: 20μm. B, similar number of PH3+ EGFP+ cells between WT and mutants in 425μm long regions of the spinal cord 36–50 hpf. Data from N = 27 WT and N = 9 mutants (36hpf) and N = 15 WT and N = 10 mutants (50hpf). C, HB9 immunostained 50hpf larvae to label motor neurons. Scale bar: 12.5μm. D, similar number of motor neurons between WT and mutant in 150μm long regions of the spinal cord. Data from N = 13 WT and N = 9 mutants. Error bars indicate ± SD.