FIGURE

Fig. 9

ID

ZDB-FIG-161116-17

Publication

Almeida et al., 2016 - Oligodendrocyte Development in the Absence of Their Target Axons In Vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 9

Impaired survival of a subset of oligodendrocyte-lineage cells in the posterior spinal cord of kbp mutants.

A, High-magnification live-imaging of the posterior spinal cord of 72hpf Tg(sox10:mRFP) larvae, with Mauthner axon myelin sheaths in WTs (pink bracket) and mRFP+ debris in mutants (pink arrowheads). Scale bars: 10 μm B, Posterior spinal cord of 72hpf Tg(sox10:mRFP) larvae stained with Acridine Orange; mutants have AO+ foci (arrowheads). See text for details. Scale bar: 20 μm. C, Time-lapse stills showing a mutant oligodendrocyte forming a myelin sheath (brackets) and then dying, originating mRFP+ debris (arrowheads). Scale bar: 10μm. D, Proportion of larvae with oligodendrocyte death events: essentially only mutants show any events, only in the posterior spinal cord. E, Rate of oligodendrocyte (OL) death (per 12-hour) is significantly elevated only in the posterior spinal cord of kbp mutants. Data from N = 18 WT and N = 14 mutants (anterior) and N = 18WT and N = 21 mutants (posterior). * indicates P<0.05; see text for details. Error bars indicate ± SD.

Expression Data

Genes:	EGFP mCherry
Fish:	vu234Tg vu12Tg; vu234Tg kifbp^st23/st23; vu12Tg; vu234Tg kifbp^st23/st23; vu234Tg
Anatomical Terms:	Mauthner neuron myelin sheath oligodendrocyte cytoplasm oligodendrocyte membrane
Stage Range:	Pec-fin to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kifbp^st23/st23; vu12Tg; vu234Tg kifbp^st23/st23; vu234Tg
Observed In:	oligodendrocyte cell death
Stage Range:	Pec-fin to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One