Fig. 4

Gfi1aa expression in haemogenic endothelial cells is induced downstream of VegfA and Notch signaling, but independent of Runx1. Fixed qmc551;gata1:dsRed double transgenic embryos after GFP/dsRed immunohistochemistry are shown in (A, D-E, I). Fixed wt, qmc551 and qmc551;gata1:dsRed double transgenic embryos stained by WISH are shown in (B-C, H, K), (L) and (J), respectively. Live qmc551 embryos that were wt, heterozygous or homozygous mib carriers were imaged in (F-G). Confocal images of optical sagittal sections through the DA are 1.6 and 0.995 µm (A), 6.5 and 6.6 µm (D), 1.2 µm (E) and 2.7 µm (I) thick. A confocal maximum intensity projection of a 37 µm optical slice is shown in (G). Embryos were treated with DMSO, the VegfR inhibitors 676,475 (A) and SU5416 (B,C) or DAPM (D) from tailbud stage (10 hpf). Rbpja/b (E) and runx1 (I-L) morpholinos were injected at 2–4 cell stage. PrRBCs, HECs and inner ear hair cells are labeled with red, green and yellow arrows, respectively. Arrowheads mark reduced or absent staining. Fractions x/y give the number of embryos, x, with depicted phenotype out of all embryos analyzed, y. Embryos are shown with anterior left and dorsal up.