FIGURE

Fig. 3

ID

ZDB-FIG-160831-5

Publication

Song et al., 2016 - p53-Dependent DNA damage response sensitive to editing-defective tRNA synthetase in zebrafish

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Fig. 3

ED ValRS activates p53 in zebrafish. (A) RT-PCR results of p53 downstream markers p21, gadd45, and p53R2 in ValRS^WT or ValRS^ED mRNA-injected zebrafish. The bar graphs show the mean values ± SEM after normalization to the β-actin level. (B) Uninjected (n = 118), ValRS^WT mRNA-injected (n = 90), or ValRS^ED mRNA-injected (n = 93) p53^zdf1/zdf1 zebrafish were stained with AO at 1 dpf. The red arrows show points of cell death. Zebrafish are aligned based on cell death severity. (Scale bar, 500 µm.) (C) Quantification of AO staining. Bars represent mean ± SEM. (D) RT-PCR results of p53 downstream markers p21, gadd45, and p53R2 in p53^zdf1/zdf1 zebrafish injected with either ValRS^WT or ValRS^ED mRNA. The bar graphs show the mean values ± SEM after normalization to the β-actin level.

Expression Data

Genes:	cdkn1a gadd45aa rrm2b
Fish:	AB tp53^zdf1/zdf1
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA