ZFIN Image: Song et al., 2016, Fig. 3

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Figure Caption

Fig. 3

ED ValRS activates p53 in zebrafish. (A) RT-PCR results of p53 downstream markers p21, gadd45, and p53R2 in ValRS^WT or ValRS^ED mRNA-injected zebrafish. The bar graphs show the mean values ± SEM after normalization to the β-actin level. (B) Uninjected (n = 118), ValRS^WT mRNA-injected (n = 90), or ValRS^ED mRNA-injected (n = 93) p53^zdf1/zdf1 zebrafish were stained with AO at 1 dpf. The red arrows show points of cell death. Zebrafish are aligned based on cell death severity. (Scale bar, 500 µm.) (C) Quantification of AO staining. Bars represent mean ± SEM. (D) RT-PCR results of p53 downstream markers p21, gadd45, and p53R2 in p53^zdf1/zdf1 zebrafish injected with either ValRS^WT or ValRS^ED mRNA. The bar graphs show the mean values ± SEM after normalization to the β-actin level.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA