FIGURE

Fig. S7

ID

ZDB-FIG-160706-37

Publication

Kwon et al., 2016 - In vivo modulation of endothelial polarization by Apelin receptor signalling

Other Figures

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Fig. S7

apln mutants show reduced endothelial cell numbers.

(a-b) in situ hybridization of 48 hpf wild-type (a) and apln^-/- (b) embryos for apela expression. The numbers of embryos examined are indicated in the top right corner of the images. (c, d) Quantitative analysis of EC numbers per three somites in 72 hpf aplnrb^+/- (c) and apln ^-/- (d) larvae. The numbers of larvae (n) and ECs (n′) are indicated above the graph. (e-f′) Confocal images (lateral views) of 60 hpf Tg(fli1a:EGFP); Tg(-0.8flt1:RFP) wild-type (e, e′) and aplnrb^+/- (f, f′) embryos. (g-i) in situ hybridization of 48 hpf wild-type (g), aplnrb^+/- (h) and aplnrb^-/- (i) embryos for vegfr3 expression. (j-k′) Confocal images (lateral views) of 60 hpf Tg(fli1a:EGFP); Tg(EPV.TP1-Mmu.Hbb:hist2h2l97 mCherry) wild-type (j, j′) and aplnrb^+/- (k, k′) embryos. The numbers of embryos examined are in the top right corner of the images (e′, f′, g-i, j′, k′). Anterior to t 98 he left, dorsal to the top. Scale bars, 60 µm (a-b, g-i) , 20 µm (e-f′, j-k′). DA, dorsal aorta; PCV, posterior cardinal vein. Error bars, SEM.

Expression Data

Genes:	apela EGFP flt4 mCherry RFP
Fish:	AB apln^mu267/mu267 aplnrb^mu270/mu270 aplnrb^mu270/+ hu5333Tg; y1Tg s939Tg; y1Tg aplnrb^mu270/+; hu5333Tg; y1Tg aplnrb^mu270/+; s939Tg; y1Tg
Anatomical Terms:	posterior cardinal vein vasculature whole organism
Stage Range:	Long-pec to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	aplnrb^mu270/mu270 aplnrb^mu270/+
Observed In:	endothelial cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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