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Fig. S7
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Fig. S7
apln mutants show reduced endothelial cell numbers.
(a-b) in situ hybridization of 48 hpf wild-type (a) and apln -/- (b) embryos for apela expression. The numbers of embryos examined are indicated in the top right corner of the images. (c, d) Quantitative analysis of EC numbers per three somites in 72 hpf aplnrb+/- (c) and apln -/- (d) larvae. The numbers of larvae (n) and ECs (n′) are indicated above the graph. (e-f′) Confocal images (lateral views) of 60 hpf Tg(fli1a:EGFP); Tg(-0.8flt1:RFP) wild-type (e, e′) and aplnrb+/- (f, f′) embryos. (g-i) in situ hybridization of 48 hpf wild-type (g), aplnrb +/- (h) and aplnrb -/- (i) embryos for vegfr3 expression. (j-k′) Confocal images (lateral views) of 60 hpf Tg(fli1a:EGFP); Tg(EPV.TP1-Mmu.Hbb:hist2h2l97 mCherry) wild-type (j, j′) and aplnrb+/- (k, k′) embryos. The numbers of embryos examined are in the top right corner of the images (e′, f′, g-i, j′, k′). Anterior to t 98 he left, dorsal to the top. Scale bars, 60 µm (a-b, g-i) , 20 µm (e-f′, j-k′). DA, dorsal aorta; PCV, posterior cardinal vein. Error bars, SEM.
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Full text @ Nat. Commun.