FIGURE

Fig. 1

ID

ZDB-FIG-160517-19

Publication

Hsu et al., 2015 - A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect.

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Fig. 1

The new alleles show similar Notch molecular phenotypes to those of previous mib mutants.All embryos were raised at 22 °C and flat-mounted WISH performed at 22 hpf. A-D are flat-mounted her4 WISH. (A) WT embryos expressed her4 in brain and neural tube; (B) mibⁿⁿ²⁰⁰⁰, (C) mibⁿⁿ²⁰⁰¹ and (D) mibⁿⁿ²⁰⁰² mutants all showed a down-regulation of her4 expression. E-H are flat-mounted huc WISH. (E) WT embryos expressed huc in cells of brain and neural tube; (F) mibⁿⁿ²⁰⁰⁰, (G) mibⁿⁿ²⁰⁰¹ and (H) mibⁿⁿ²⁰⁰² mutants all showed an up-regulation of huc expression. I-L are flat-mounted dlc WISH. (I) WT embryos expressed dlc in PSM, formed somites and cell clusters in the hindbrain; (J) mibⁿⁿ²⁰⁰⁰, (K) mibⁿⁿ²⁰⁰¹ and (L) mibⁿⁿ²⁰⁰² mutants all showed a salt-and-pepper dlc pattern in the nascent somites and anterior PSM. Note that the dlc expression in formed somites was lost in (L) mibⁿⁿ²⁰⁰² mutants.

Expression Data

Genes:	dlc elavl3 her4.1
Fish:	WT mib1^{nn2000/nn2000} mib1^{nn2001/nn2001} Df(Chr2:mib1,foxc1a,rab18b,yme1l1b,gata6,cables1,foxc1a,cldn1,znfl2a,dvl3a,selenot1a))nn2002/nn2002
Condition:	cold exposure
Anatomical Terms:	brain hindbrain cell neural tube segmental plate somite
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mib1^{nn2000/nn2000} mib1^{nn2001/nn2001} Df(Chr2:mib1,foxc1a,rab18b,yme1l1b,gata6,cables1,foxc1a,cldn1,znfl2a,dvl3a,selenot1a))nn2002/nn2002
Condition:	cold exposure
Observed In:	brain neural tube segmental plate somite
Stage:	26+ somites

Phenotype Detail

Acknowledgments

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